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Identification and functional enrichment of ferroptosis-related differentially expressed genes in the GSE36791 peripheral-blood dataset. (A) Heatmap of all differentially expressed genes in control and aSAH samples. (B) Heatmap of the 35 ferroptosis-related differentially expressed genes. (C) Venn diagrams showing the overlap between all genes detected in GSE36791 and human ferroptosis-related genes obtained from FerrDb, and the overlap between GSE36791 differentially expressed genes and FerrDb genes, identifying 35 ferroptosis-related differentially expressed genes. (D) Spearman correlation heatmap of the 35 ferroptosis-related differentially expressed genes. (E) Gene Ontology enrichment analysis of these genes. (F) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of these genes. DEGs, differentially expressed genes; Ferr-DEGs, ferroptosis-related differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.
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Identification and functional enrichment of ferroptosis-related differentially expressed genes in the GSE36791 peripheral-blood dataset. (A) Heatmap of all differentially expressed genes in control and aSAH samples. (B) Heatmap of the 35 ferroptosis-related differentially expressed genes. (C) Venn diagrams showing the overlap between all genes detected in GSE36791 and human ferroptosis-related genes obtained from FerrDb, and the overlap between GSE36791 differentially expressed genes and FerrDb genes, identifying 35 ferroptosis-related differentially expressed genes. (D) Spearman correlation heatmap of the 35 ferroptosis-related differentially expressed genes. (E) Gene Ontology enrichment analysis of these genes. (F) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of these genes. DEGs, differentially expressed genes; Ferr-DEGs, ferroptosis-related differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.
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Identification and functional enrichment of ferroptosis-related differentially expressed genes in the GSE36791 peripheral-blood dataset. (A) Heatmap of all differentially expressed genes in control and aSAH samples. (B) Heatmap of the 35 ferroptosis-related differentially expressed genes. (C) Venn diagrams showing the overlap between all genes detected in GSE36791 and human ferroptosis-related genes obtained from FerrDb, and the overlap between GSE36791 differentially expressed genes and FerrDb genes, identifying 35 ferroptosis-related differentially expressed genes. (D) Spearman correlation heatmap of the 35 ferroptosis-related differentially expressed genes. (E) Gene Ontology enrichment analysis of these genes. (F) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of these genes. DEGs, differentially expressed genes; Ferr-DEGs, ferroptosis-related differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Journal: Frontiers in Neurology

Article Title: Ferroptosis-associated candidate genes in aneurysmal subarachnoid hemorrhage: evidence from human peripheral blood and mouse brain

doi: 10.3389/fneur.2026.1823039

Figure Lengend Snippet: Identification and functional enrichment of ferroptosis-related differentially expressed genes in the GSE36791 peripheral-blood dataset. (A) Heatmap of all differentially expressed genes in control and aSAH samples. (B) Heatmap of the 35 ferroptosis-related differentially expressed genes. (C) Venn diagrams showing the overlap between all genes detected in GSE36791 and human ferroptosis-related genes obtained from FerrDb, and the overlap between GSE36791 differentially expressed genes and FerrDb genes, identifying 35 ferroptosis-related differentially expressed genes. (D) Spearman correlation heatmap of the 35 ferroptosis-related differentially expressed genes. (E) Gene Ontology enrichment analysis of these genes. (F) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of these genes. DEGs, differentially expressed genes; Ferr-DEGs, ferroptosis-related differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies: anti-PGD (1:100, Cat. A0563, Abclonal, Wuhan, China), anti-STAT3 (1:100, Cat. A11216, Abclonal), anti-MAPK3/ERK1 (1:100, Cat. A19561, Abclonal), and anti-G6PD (1:100, Cat. A27296, Abclonal).

Techniques: Functional Assay, Control, Analysis

Network-based identification of ferroptosis-associated candidate hub genes in aSAH. (A) PPI network constructed from ferroptosis-related DEGs. (B) Top-ranked PPI hub genes identified by cytoHubba. (C–E) MCODE clusters identified from the PPI network, with scores of 4.286, 4.000, and 3.000, respectively. (F) Intersection between WGCNA module genes and PPI-derived hub genes. (G) Overlap between the four selected hub genes and DCI-associated genes. PPI, protein–protein interaction; DCI, delayed cerebral ischemia.

Journal: Frontiers in Neurology

Article Title: Ferroptosis-associated candidate genes in aneurysmal subarachnoid hemorrhage: evidence from human peripheral blood and mouse brain

doi: 10.3389/fneur.2026.1823039

Figure Lengend Snippet: Network-based identification of ferroptosis-associated candidate hub genes in aSAH. (A) PPI network constructed from ferroptosis-related DEGs. (B) Top-ranked PPI hub genes identified by cytoHubba. (C–E) MCODE clusters identified from the PPI network, with scores of 4.286, 4.000, and 3.000, respectively. (F) Intersection between WGCNA module genes and PPI-derived hub genes. (G) Overlap between the four selected hub genes and DCI-associated genes. PPI, protein–protein interaction; DCI, delayed cerebral ischemia.

Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies: anti-PGD (1:100, Cat. A0563, Abclonal, Wuhan, China), anti-STAT3 (1:100, Cat. A11216, Abclonal), anti-MAPK3/ERK1 (1:100, Cat. A19561, Abclonal), and anti-G6PD (1:100, Cat. A27296, Abclonal).

Techniques: Construct, Derivative Assay